Transgenerational Changes in Somatic And Germ Line Genetic Integrity of First-Generation offspring Derived from the DNA Damaged Sperm

Adiga, Satish Kumar and Upadhya, Dinesh and Kalthur, Guruprasad and Rao, Satish BS and Kumar, Pratap (2009) Transgenerational Changes in Somatic And Germ Line Genetic Integrity of First-Generation offspring Derived from the DNA Damaged Sperm. Fertility and Sterility. pp. 2-4.

PDF Adiga_2009_Fertility-and-Sterility.pdf - Published Version Restricted to Registered users only Download (363kB) | Request a copy

Abstract

Objective: To report a more quantitative approach to study the influence of varying levels of sperm DNA damage on transgenerational changes in genomic instability in a mouse model. Design: Experimental prospective study. Setting: Embryology research laboratory. Animal(s): Swiss Albino mice. Intervention(s): The sperm DNA damage was induced by different doses of g-irradiation to male mice followed by mating with healthy female mice. Main Outcome Measure(s): Genomic integrity in embryos, fetus, and spermatozoa of F1 mice derived from the DNA-damaged sperm. Result(s): The transgenerational changes in genetic integrity were attributed by a dose-dependent increase in the frequency of micronuclei in preimplantation embryos and a concomitant increase in genomic instability in fetal liver cells and sperm chromatin modifications in F1 males. A strong positive correlation was observed between the extent of sperm DNA damage and somatic and germ-line genomic instability. Conclusion(s): Sperm-mediated transgenerational genomic instability is dependent on the amount of DNA damage present in the sire’s sperm at the time of fertilization.

Actions (login required)

View Item