Adiga, Satish Kumar and Upadhya, Dinesh and Kalthur, Guruprasad and Rao, Satish BS and Kumar, Pratap (2009) Transgenerational Changes in Somatic And Germ Line Genetic Integrity of First-Generation offspring Derived from the DNA Damaged Sperm. Fertility and Sterility. pp. 2-4.
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Abstract
Objective: To report a more quantitative approach to study the influence of varying levels of sperm DNA damage on transgenerational changes in genomic instability in a mouse model. Design: Experimental prospective study. Setting: Embryology research laboratory. Animal(s): Swiss Albino mice. Intervention(s): The sperm DNA damage was induced by different doses of g-irradiation to male mice followed by mating with healthy female mice. Main Outcome Measure(s): Genomic integrity in embryos, fetus, and spermatozoa of F1 mice derived from the DNA-damaged sperm. Result(s): The transgenerational changes in genetic integrity were attributed by a dose-dependent increase in the frequency of micronuclei in preimplantation embryos and a concomitant increase in genomic instability in fetal liver cells and sperm chromatin modifications in F1 males. A strong positive correlation was observed between the extent of sperm DNA damage and somatic and germ-line genomic instability. Conclusion(s): Sperm-mediated transgenerational genomic instability is dependent on the amount of DNA damage present in the sire’s sperm at the time of fertilization.
Item Type: | Article |
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Uncontrolled Keywords: | Sperm DNA damage, Preimplantation embryos, Genomic instability, Transgenerational effect |
Subjects: | Medicine > KMC Manipal > Clinical Embryology Life Sciences > MLSC Manipal |
Depositing User: | KMC Manipal |
Date Deposited: | 20 Aug 2011 09:17 |
Last Modified: | 17 Aug 2021 10:11 |
URI: | http://eprints.manipal.edu/id/eprint/1149 |
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