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Influence of Vindesine Exposure on The Micronucleus Formation And Cell Survival in V79 Cells

Jagetia, Ganesh Chandra and Adiga, Satish Kumar (1998) Influence of Vindesine Exposure on The Micronucleus Formation And Cell Survival in V79 Cells. Mutation Research, 421. pp. 55-63.

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Abstract

Effect of different concentrations (0, 1, 5, 10, 20 and 50 nM) of vindesine sulphate was studied on clonogenicity and micronucleus �MN. formation in V79 (Chinese hamster lung fibroblasts. cells. Exposure of V79 cells to vindesine for 6 h resulted in a concentration dependent decline in cell survival. The frequency of micronuclei (MN) increased in a concentration dependent manner at 16, 22 and 28 h post-exposure. The frequency of MN increased significantly after 5 to 50 nM drug exposure at 16 and 22 h post-treatment, while increasing post-exposure time to 28 h resulted in a significant increase in MN frequency at all exposure doses of vindesine. The statistical evaluation of concurrent concentrations at various time periods showed a non-significant difference in MN frequency among various post-exposure time periods, except 16 h and 28 h for 50 nM, where a significant decline in the MN frequency was observed at 28 h compared to 16 h post-exposure. The cell proliferation indices showed a concentration dependent decline in the frequency of binucleate cells and this decline was linear quadratic. The increasing drug concentration resulted in a concentration dependent decline in cell survival. While the frequency of micronuclei increased the cell survival decreased and the relationship between cell survival and micronucleus induction was linear quadratic.

Item Type: Article
Uncontrolled Keywords: Vindesine, Survival, Micronucleus, V79 cell
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 22 Aug 2011 04:25
Last Modified: 22 Aug 2011 04:25
URI: http://eprints.manipal.edu/id/eprint/1163

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