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Correlation Between Cell Survival And Micronuclei Formation in V79 cells Treated with Vindesine Before Exposure to Different Doses of G-Radiation

Jagetia, Ganesh Chandra and Adiga, Satish Kumar (2000) Correlation Between Cell Survival And Micronuclei Formation in V79 cells Treated with Vindesine Before Exposure to Different Doses of G-Radiation. Mutation Research, 448. pp. 57-68.

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Abstract

Effect of 20 nM vindesine sulphate (VDS) treatment was studied on cell survival, growth kinetics and micronuclei induction in V79 cells exposed to 0–300 cGy of g-radiation at 16, 22 and 28 h post-irradiation. Treatment of V79 cells with VDS before exposure to different doses of gamma radiation resulted in a significant decline in cell survival and growth kinetic when compared with the concurrent PBSqirradiation group. The decline in cell survival and growth kinetics was dose related. Similarly, the cell proliferation indices also declined in a dose dependent manner in both PBSqirradiation and VDSqirradiation groups and this decline was higher in VDSqirradiation group in comparison with the PBSqirradiation group. In contrast, the frequency of micronuclei increased in a dose related manner in both PBSqirradiation and VDSqirradiation groups. However, the frequency of micronuclei was significantly greater in the VDSqirradiation group when compared to the PBSqirradiation group at all the post-irradiation time periods studied and the dose response for both groups was linear for all the scoring time periods. The biological response was determined by plotting surviving fraction and micronuclei frequencies on X- and Y-axes, respectively. The plot between surviving fraction and micronuclei induction showed a close correlation. The surviving fraction of V79 cells reduced with the increasing frequency of micronuclei in both groups and the relationship between micronuclei induction and cell survival could be fitted on a linear quadratic model.

Item Type: Article
Uncontrolled Keywords: V79 cells, Vindesine, Radiation, Survival, Micronuclei, Cell proliferation index
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Manipal
Date Deposited: 22 Aug 2011 04:13
Last Modified: 22 Aug 2011 04:13
URI: http://eprints.manipal.edu/id/eprint/1164

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