Preventive Efficacy of Hydroalcoholic Extract of Cymbopogon Citratus Against Radiation-Induced DNA Damage on V79 Cells and Free Radical Scavenging Ability Against Radicals Generated in Vitro

Rao, Satish BS and Shanbhoge, R and Rao, BN and Adiga, Satish Kumar and Upadhya, D and Aithal, BK and Kumar, MRS (2009) Preventive Efficacy of Hydroalcoholic Extract of Cymbopogon Citratus Against Radiation-Induced DNA Damage on V79 Cells and Free Radical Scavenging Ability Against Radicals Generated in Vitro. Human & Experimental Toxicology, 28. pp. 195-202.

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Abstract

This study presents the findings of free radical scavenging and antigenotoxic effect of hydroalcoholic extract of Cymbopogon citratus (CCE). The CCE at a concentration of 60 μg/mL resulted in a significant scavenging ability of 2,2-diphenyl-2-picryl hydrazyl (DPPH; (85%), 2,2- azinobis (3-ethyl benzothiazoline-6-sulphonic acid) (ABTS; 77%), hydroxyl (70%), superoxide (76%), nitric oxide (78%) free radicals generated using in vitro and also a moderate anti-lipid peroxidative effect (57%). Further, the radiation-induced antigenotoxic potential of CCE was assessed in Chinese hamster lung fibroblast cells (V79) using micronucleus assay. The CCE resulted in a dose-dependent decrease in the yield of radiationinduced micronuclei, with a maximum effect at 125 μg/ mL CCE for 1 h before 2 Gy of radiation. Similarly, there was a significant (P < 0.05–0.0001) decrease in percentage of micronuclei when V79 cells were treated with optimal dose of CCE (125 μg/mL) before exposure to different doses of gamma radiation, that is, 0.5–4 Gy, compared with radiation alone groups. The results of the micronucleus study indicated antigenotoxic effect demonstrating the radioprotective potential of CCE and, which may partly due to its and antioxidant capacity as it presented its ability to scavenge various free radicals in vitro and anti-lipid peroxidative potential.

Item Type: Article
Uncontrolled Keywords: Antioxidant, Cymbopogon citratus, Free radical scavenger, Micronuclei, Radioprotection
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Manipal
Date Deposited: 23 Aug 2011 04:23
Last Modified: 28 Oct 2016 09:44
URI: http://eprints.manipal.edu/id/eprint/1169

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