Delayed And Stage Specific Phosphorylation of H2AX During Preimplantation Development of g-irradiated Mouse Embryos

Adiga, Satish Kumar and Toyoshima, Megumi and Shimura, Tsutomu and Takeda, Jun and Uematsu, Norio and Niwa, Ohtsura (2007) Delayed And Stage Specific Phosphorylation of H2AX During Preimplantation Development of g-irradiated Mouse Embryos. Reproduction, 133 (2). pp. 415-422.

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Abstract

Within minutes of the induction of DNA double-strand breaks in somatic cells, histone H2AX becomes phosphorylated in the serine 139 residue at the damage site. The phosphorylated H2AX, designated as g-H2AX, is visible as nuclear foci in the irradiated cells which are thought to serve as a platform for the assembly of proteins involved in checkpoint response and DNA repair. It is known that early stage mammalian embryos are highly sensitive to radiation but the mechanism of radiosensitivity is not well understood. Thus, we investigated the damage response of the preimplantation stage development by analyzing focus formation of g-H2AX in mouse embryos g-irradiated in utero. Our analysis revealed that although H2AX is present in early preimplantation embryos, its phosphorylation after 3 Gy g-irradiation is hindered up to the two cell stage of development.When left in utero for another 24–64 h, however, these irradiated embryos showed delayed phosphorylation of H2AX. In contrast, phosphorylation of H2AX was readily induced by radiation in post-compaction stage embryos. It is possible that phosphorylation of H2AX is inefficient in early stage embryos. It is also possible that the phosphorylated H2AX exists in the dispersed chromatin structure of early stage embryonic pronuclei, so that it cannot readily be detected by conventional immunostaining method. In either case, this phenomenon is likely to correlate with the lack of cell cycle arrest, apoptosis and high radiosensitivity of these developmental stages.

Item Type: Article
Subjects: Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Manipal
Date Deposited: 23 Aug 2011 04:05
Last Modified: 23 Aug 2011 04:05
URI: http://eprints.manipal.edu/id/eprint/1170

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