MU Digital Repository
Logo

Correlation Between Cell Survival, Micronuclei-Induction, and LDH Activity in V79 Cells Treated with Teniposide (VM-26) Before Exposure to Different Doses of g Radiation

Adiga, Satish Kumar and Jagetia, Ganesh Chandra (1999) Correlation Between Cell Survival, Micronuclei-Induction, and LDH Activity in V79 Cells Treated with Teniposide (VM-26) Before Exposure to Different Doses of g Radiation. Toxicology Letters, 109. pp. 31-41.

[img] PDF
Toxicol_letters-Adiga_&_Jagetia-1999.pdf - Published Version
Restricted to Registered users only

Download (117kB) | Request a copy

Abstract

The influence of teniposide (VM-26) treatment was studied on the radiation-induced alterations in cell survival, micronuclei (MN) formation and lactate dehydrogenase (LDH) release in V79 cells. Treatment of V79 cells with 10 nM teniposide before exposure to different doses of g radiation resulted in a significant decline in the cell survival when compared with the PBS�irradiation group. The decline in cell survival was dose related. The cell proliferation indices also declined in a dose-dependent manner in both PBS�irradiation and VM-26�irradiation groups. The decline was higher in the VM-26�irradiaion group in comparison with the PBS�irradiation group. In contrast, the frequency of micronuclei increased in a dose-related manner in both PBS�irradiation and VM-26�irradiation groups. However, the frequency of micronuclei was significantly greater in the latter group when compared with the former group at all the post-irradiation time periods studied. The LDH contents increased in a dose-dependent manner in both PBS�irradiation and VM-26�irradiation groups at all the post-irradiation time periods evaluated. This elevation in LDH contents was significantly greater in the VM-26�irradiation group in comparison with the PBS�irradiation group. © 1999 Elsevier Science Ireland Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Teniposide, Radiation, V79 cells, Survival, Micronuclei;, Lactate dehydrogenase
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 23 Aug 2011 04:00
Last Modified: 23 Aug 2011 04:00
URI: http://eprints.manipal.edu/id/eprint/1171

Actions (login required)

View Item View Item