Effect of Teniposide (VM-26) on the Cell Survival, Micronuclei-Induction And Lactate Dehydrogenase Activity on V79 Cells

Adiga, Satish Kumar and Jagetia, Ganesh Chandra (1999) Effect of Teniposide (VM-26) on the Cell Survival, Micronuclei-Induction And Lactate Dehydrogenase Activity on V79 Cells. Toxicology, 138. pp. 29-41.

PDF Toxicology-Adiga_&_Jagetia-1999.pdf - Published Version Restricted to Registered users only Download (176kB) | Request a copy

Abstract

The genotoxic effect of 0, 1, 5, 10, 25, 50 and 100 nM teniposide (VM-26) treatment was studied on cultured V79 cells. Treatment of V79 cells with different concentrations of teniposide resulted in a concentration-dependent decline in the cell survival and growth kinetics. VM-26 treatment also caused alteration in the cell proliferation kinetics as evidenced by the increase in the frequency of mononucleate cells, with a consequent decline in the frequency of binucleate cells in a concentration-dependent manner at all the post-treatment time periods. Exposure of V79 cells to different concentrations of VM-26 resulted in a concentration related elevation in the frequency of micronucleated binucleate (MN) cells. The frequency of MN was significantly higher in VM-26 treated cells than that of non-drug treated cells at all the post-treatment time periods. A peak frequency of MN was observed at 16 h post-treatment that declined thereafter. The release of lactate dehydrogenase increased with the increase in drug concentration and a maximum LDH release was observed at 0.5 h post-treatment after exposure to 10–100 nM VM-26. While a peak value was observed at 1 and 2 h for 5 and 1 nM VM-26, respectively. The biological response was evaluated by determining the relationship between micronuclei and cell survival. The cell survival declined with increasing MN frequency, resulting in a close but an inverse relationship between the cell survival and micronuclei-induction. The dose effect relationships for micronuclei induction, LDH release and biological response was linear quadratic. © 1999 Elsevier Science Ireland Ltd. All rights reserved.

Actions (login required)

View Item