A Comparative study of in Vivo efficacy of currently used Antibiotics in Murine model of Klebsiella pneumonia

PAI, PREETHI G and *, ANOOP CH and *, MIRSHAD PV and BHAT, GOPALKRISHNA and SHENOY, SHALINI M and *, KV RAMESH (2010) A Comparative study of in Vivo efficacy of currently used Antibiotics in Murine model of Klebsiella pneumonia. Asian Journal of Pharmaceutical and Clinical Research, 3 (3). pp. 224-228. ISSN 0974-2441

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Abstract

A comparative in-vivo efficacy of commonly used antibiotics was evaluated in the murine model of Klebsiella pneumonia. Six groups of immunosuppressed (cyclophosphamide 80mg/kg i.p. on -4 and -1 day) female Wistar rats (n=6-12) were employed to induce pneumonia. Bacterial inoculum of strength 1x 107 CFU/ml of Klebsiella pneumoniae strain isolated from sputum in the Department of Microbiology, Kasturba Medical College, Mangalore was injected intratracheally. Gentamicin (0.032g/kg body weight/day), Levofloxacin (0.036g/kg body weight/12hrly), Cefepime (0.18 g/kg body weight/12hrly), Aztreonam (0.18g/kg body weight/8hrly) and Meropenem (0.09 mg/kg body weight/8hrly) were administered i.p. within 4 hours following bacterial inoculation and continued for 5 days. On the sixth day, rats were sacrificed and the pneumonic lung tissues were used for bacterial count and histopathological studies. Levofloxacin significantly offered protection in Klebsiella pneumonia. Aztreonam seems to be a better choice to Cefepime and Gentamicin. Cefepime is found to be an effective antibiotic whereas Gentamicin is not effective against the local strains of K.pneumoniae. Thus in-vivo efficacy of antibiotic data may help to substantiate the choice of antibiotic against local strains of pneumopathogens. Also, these results may ensure more scientific authenticity than in-vitro bacteriological investigation reports

Item Type: Article
Uncontrolled Keywords: In vivo efficacy of antibiotics, Klebsiella pneumoniae, Pneumonia
Subjects: Medicine > KMC Mangalore > Microbiology
Medicine > KMC Mangalore > Pharmacology
Depositing User: KMCMLR User
Date Deposited: 12 Sep 2011 04:53
Last Modified: 03 Feb 2012 09:42
URI: http://eprints.manipal.edu/id/eprint/1300

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