In vitro scanning electron microscopic study on the effect of doxycycline and vancomycin on enterococcal induced biofilm

Somayaji, Krishnaraj and Acharya, Shashirashmi and Bairy, Indira and Peralam Yegneswaran, Prakash and Rao, Sakkattu Muddanna and Ballal, Vasudev (2010) In vitro scanning electron microscopic study on the effect of doxycycline and vancomycin on enterococcal induced biofilm. IEJ, 5 (2). pp. 53-58.

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Abstract

Enterococcus (E) faecalis bacteria adhere to dentine of teeth root canals to formthe biofilm. E. faecalis has been shown to be resistant to antibiotics. This in vitro study aimed toinvestigatethe efficacy of vancomycin and doxycycline in inhibiting E. faecalis biofilm formation. Materials and Methods: A total of 60 extracted human teeth were incubated with E. faecalis (ATCC 35550 strain) for 45 days to allow biofilm formation. The teeth were equally divided into six groups (n=10): 1) positive control, 2) negative control, 3) doxycycline alone 4) doxycycline with filing, 5) vancomycin alone, 6) vancomycin with filing. The relevant canals were irrigated with 4μg/mL of either vancomycin or doxycycline antibiotic. Teeth were processed for scanning electron microscopy (SEM). Areas of biofilm remaining in the canals after antibiotic treatment were measured with Scion image analysis software using the SEM images.

Item Type: Article
Uncontrolled Keywords: Biofilm, Doxycycline, Enterococcus faecalis, Root canal, Vancomycin
Subjects: Dentistry > MCODS Manipal > Conservative Dentistry and Endontics
Medicine > KMC Manipal > Microbiology
Depositing User: KMC Manipal
Date Deposited: 06 Jun 2011 09:39
Last Modified: 14 Dec 2015 14:40
URI: http://eprints.manipal.edu/id/eprint/133

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