Antigen identification using skin deficient in basement-membraneprotein: a novel tool for the diagnosis of subepidermal immunobullous diseases

Rao, R (2012) Antigen identification using skin deficient in basement-membraneprotein: a novel tool for the diagnosis of subepidermal immunobullous diseases. Clinical and Experimental Dermatology. pp. 1-6.

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Abstract

Common unifying features of the subepidermal blistering diseases are the presence of tense blisters clinically and demonstration by immunofluorescence of linear deposition of immunoreactants along the dermoepidermal junction. Further characteriza-tion of subtype is possible by identification of the target antigen by immunoblotting. However, immunoblotting is time-consuming and may not be practical for routine use in the laboratory. In this report, we describe a simple technique to identify the target antigen by indirect immunofluorescence, using epidermolysis bullosa skin as a substrate.

Item Type: Article
Subjects: Medicine > KMC Manipal > Dermatology
Depositing User: KMC Manipal
Date Deposited: 29 May 2013 06:20
Last Modified: 29 May 2013 06:20
URI: http://eprints.manipal.edu/id/eprint/136158

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