Sesamol prevents doxorubicin-induced oxidative damage and toxicity on H9c2 cardiomyoblasts

Nayak, Pawan G and Paul, Piya and Bansal, Punit and Kutty, Gopalan N and Pai, Sreedhara Ranganath Karkala (2013) Sesamol prevents doxorubicin-induced oxidative damage and toxicity on H9c2 cardiomyoblasts. Journal of Pharmacy and Pharmacology, 65 (7). pp. 1083-1093.

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Abstract

Objectives Exposure to toxicants like doxorubicin (Dox) damages cellular components by generating reactive oxygen species (ROS). This can be attenuated using free radical scavengers and/or antioxidants. Methods Dox-exposed cardiac myoblasts (H9c2 cells) were treated with sesamol (12.5, 25 and 50 mm), a natural phenolic compound. Intracellular ROS inhibition, cell viability and analysis of antioxidant and biochemical markers such as superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase, reduced/oxidized glutathione, lipid peroxidation and protein carbonyl content were performed. The effect of sesamol treatment on the cytotoxic and genotoxic parameters was studied by monitoring the signalling proteins involved in the apoptotic pathway. Key findings Dox triggered cellular and genetic damage by increasing levels of intracellular ROS, thereby decreasing cell viability and increasing apoptosis. Sesamol reversed the cytotoxic and genotoxic effects of Dox. In addition, sesamol attenuated the pro-apoptotic proteins and improved the anti-apoptotic status. Sesamol pre-treatment also alleviated the disturbed antioxidant milieu by preventing ROS production and improving endogenous enzyme levels. Conclusions Among the different doses tested, 50 mm of sesamol showed maximum protection against Dox-induced oxidative damage. This reflects the significance of sesamol in ameliorating the deleterious effects associated with cancer chemotherapy

Item Type: Article
Uncontrolled Keywords: Anti-apoptotic; Doxorubicin; Free radical; pBR322 DNA; Reactive oxygen species; Sesamol
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 14 Jun 2013 04:23
Last Modified: 07 Nov 2016 08:53
URI: http://eprints.manipal.edu/id/eprint/136307

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