Partial characterisation and therapeutic application of Protease from a fungal species

Kalaskar, VV and Narayanan, K and Subramanyam, VM and Rao, VJ (2012) Partial characterisation and therapeutic application of Protease from a fungal species. Indian Drugs, 49 (10). pp. 42-46.

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Abstract

Proteases find wide application in textile, leather, food and pharmaceutical industries. Also this enzyme has considerable therapeutic importance in treating inflammation, digestive and vascular conditions. A protease obtained from a fungal isolate, Aspergillus niger, was tested for clinical application. The microorganism was cultivated on a starch-casein medium. The proteolytic activity was assayed with casein substrate using Folin-Ciocalteu’s method having tyrosine standard. The enzyme was precipitated using 50% ammonium sulphate. Inhibition studies on EDTA, phenyl methyl sulphonyl fluoride, dithiothreitol and pepstatin A revealed that the enzyme is an acid protease. The precipitated protease exhibited significant dehairing, destaining, declotting activites on Wistar rat model. The enzyme showed prominent anti-inflammatory activity and results were compared using diclofenac sodium.

Item Type: Article
Uncontrolled Keywords: Acid protease; Aspergillus niger; Antiinflammatory activity; De-clotting activity; De-staining; De-hairing.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Biotechnology
Depositing User: KMC Manipal
Date Deposited: 25 Jun 2013 04:26
Last Modified: 25 Jun 2013 04:26
URI: http://eprints.manipal.edu/id/eprint/136367

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