Narayanan, K and Chopade, Nagesh and Raj, Vasanth P and Subrahmanyam, VM and Rao, Venkata J (2013) Fungal Chitinase Production and its Application in bio waste management. Journal of Scientific & Industrial Research, 72. pp. 393-399.

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Abstract

Chitinases find wide applications in pest control, pollution abatement and in management of marine wastes. A marine soil isolate Aspergillus terreus, having chitinase activity was cultivated on sucrose-peptone-yeast extract medium. Colloidal chitin replaced sucrose in the production medium. The cultivation conditions were optimized using single parameter approach and Placket-Burman statistical design. The enzyme activity was estimated using di-nitrosalicylic acid method. Chitinolytic activity was 52 U/ml in shake flask experiments and 29.2 U/ml on a 3 L laboratory fermenter. The enzyme extracted with cold acetone showed a prominent band having a molecular weight of 52.8 kDa on SDS PAGE. The organism utilized chitin present in mushroom (Agaricus bisporus) when used as a sole source of carbon with the enzyme activity being 82.38 U/ml. The enzyme was stable at wide temperature range 30- 70 °C maximum activity seen at 50 °C. The study has a considerable significance and impact in bioremediation of biological waste.

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