Sebastian, Sumi and Subrahmanyam, VM and Rao, Venkata J and Sivagurunathan, N and Raj, Vasanth P (2009) An invirto study on the effect of potenitiatig agents on selected fluoroquinolone antibiotics. Pharmacologyonline, 1. pp. 57-69.

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Abstract

Development of resistance to antimicrobial agents is a major problem in chemotherapy. Finding agents which potentiate antimicrobial activity could circumvent this problem. Present study was envisaged to study the in vitro action of potentiating agents viz., EDTA, caffeine, citric acid, theophylline, tartaric acid, tri-sodium citrate in combination with selected fluoroquinolone antibiotics – ciprofloxacin, sparfloxacin, levofloxacin against both Grampositive( B. subtilis, S. aureus) and Gram-negative bacteria (E. coli, P. aeruginosa). Effect of potentiating agents on antibiotics was studied by determination of MIC, zone diameter and turbimetric analysis. All potentiating agents were used in concentrations at which they do not have any antimicrobial activity. EDTA showed antibacterial activity at lower concentrations. In combination with antibiotics- EDTA, caffeine, citric acid exhibited considerable potentiation of the activity. Tri-sodium citrate exhibited least potentiation effect.

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