Estimation of Uncertainty Associated with Intensity Ratio in CDNA Microarray Experiments

Binu, VS and Nair, NS and Prasad, Manjunatha K and Kalesh, KM (2012) Estimation of Uncertainty Associated with Intensity Ratio in CDNA Microarray Experiments. Research & Reviews: A Journal of Statistics, 1 (2). pp. 24-33. ISSN 2278 – 2273

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Abstract

Microarray experiments are used to measure gene expression levels of thousands of genes simultaneously. In cDNA microarray experiments, the measurement of interest is average of red to green signal intensity ratio at each spot that gives the expression level of a particular gene in a diseased sample compared to normal. The intensity ratio for a given spot depends on many parameters and therefore uncertainty in estimating these parameters influence on uncertainty in computation of intensity ratio. This article deals with estimating uncertainty associated with the ultimate calculation of intensity ratio for each spot using the theory of error propagation. We have in fact observed that the uncertainty of intensity ratio decreases as the correlation between foreground and background pixel intensities within a spot increases.

Item Type: Article
Uncontrolled Keywords: microarray; estimation; uncertainty; covariance matrix.
Subjects: Departments at MU > Statistics
Depositing User: KMC Manipal
Date Deposited: 20 Jul 2013 04:34
Last Modified: 20 Jul 2013 04:34
URI: http://eprints.manipal.edu/id/eprint/136593

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