Salivary protein markers: a noninvasive protein profile-based method for the early diagnosis of oral premalignancy and malignancy

Patil, Ajeetkumar and Choudhari, Khoobram S and Unnikrishnan, VK and Pai, Keerthilatha M and Chidangil, Santhosh (2013) Salivary protein markers: a noninvasive protein profile-based method for the early diagnosis of oral premalignancy and malignancy. Journal of Biomedical Optics, 18 (10). pp. 101317-1. ISSN 0091-3286

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Abstract

An ultra-sensitive hyphenated technique, high-performance liquid chromatography-laser-induced fluorescence detection protein profiling of saliva, is evaluated for early detection and diagnosis of oral premalignancy and malignancy. Calibration sets of protein profiles of unstimulated whole saliva are collected from clinically/ pathologically normal, premalignant, and malignant subjects and used as standards. Three parameters—scores of factors, sum of squared residuals, and Mahalanobis distance—derived from principal component analysis of protein profiles of the standard calibration sets, and blind samples are used for “match/no-match” diagnosis of the blind samples. Analyses of the results show that the method is capable of differentiating normal, premalignant, and malignant conditions with the sensitivity and specificity of 79% and 78%, respectively. The technique provides a fast, highly objective (free from personal judgment and statistically defined), and noninvasive diagnostic method for screening and early detection of oral cancer.

Item Type: Article
Uncontrolled Keywords: Salivary proteins; oral cancer; saliva; protein profiling; high-performance liquid chromatography-laser-induced fluorescence detection; principal component analysis.
Subjects: Dentistry > MCODS Manipal > Oral Medicine and Radiology
Departments at MU > Atomic Molecular Physics
Depositing User: KMC Manipal
Date Deposited: 21 Aug 2013 04:26
Last Modified: 23 Oct 2013 09:44
URI: http://eprints.manipal.edu/id/eprint/136828

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