Khan, Saleemulla and Makhija, Inder K and Khamar, Devang and Rani, Sandhya (2010) Development and Standardization OF Turmeric Cream by HPTLC. Internatio nal Jo ur nal of Biom edical a n d Advance Research, 1 (4). pp. 109-116.

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Abstract

The aim of this study was to formulate turmeric cream and to standardize it by HPTLC using pure curcumin as a bioactive chemical marker. It was developed by incorporating extract of curcuma longa (rhizome) in a cream base by a w/o emulsification technique. Preformulation and drug-excipients in teractions were studied by HPTLC finger printing. Chromatography was performed on silica gel 60 F254 TLC plate using chloroform : ethanol: acetic acid (48:2:0.1 v/v/v ) as solvent system . Densitometry scanning was performed under reflectance-absorbance mode at 300 nm to quantify the spots. The content of curcuminoids in formulated cream was found to be 4.27 m g /g. The HPTLC method was validated as per the ICH guidelines. The proposed method is simple and sensitive and can be used for the routine assay of curcuminoids in phytome dicines containing Tumeric as an ingredient.

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