Khan, Saleemulla (2011) In Vitro α-Amylase Inhibition and Antioxidant Activities of Methanolic Extract of Amaranthus Caudatus Linn. Oman Medical Journal, 26 (3). pp. 166-170.

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Abstract

Objectives:The present study was aimed to investigate the α -amylase inhibition and antioxidant activities of methanolic extract of Amaranthus caudatus Linn (MeAc). Methods: Methanolic extract of Amaranthus caudatus was screened for α -amylase inhibition activity by CNPG3 method (2-chloro-p-nitrophenyl- α -D-maltotrioside) and antioxidant activity was evaluated by 1,1-diphenyl-2-picryl-hydrazile (DPPH) free radical scavenging, superoxide dismutase (SOD) scavenging, hydroxyl free radical scavenging, nitric oxide (NO) radical scavenging, and 2.2’-azinobis-3-ethylbenzothiazole-6-sulfonic acid (ABTS) radical scavenging assays. MeAc was also screened for non enzymatic hemoglycosylation. Results: The methanolic extract of Amaranthus caudatus showed potent α -amylase inhibition activity (IC 50 19.233 μg/ml). MeAc showed significant antioxidant activity in all the in vitro antioxidant models. Furthermore, the MeAc was found to be extremely effective in scavenging ABTS radical activity (IC 50 48.75±1.1 μg/ ml) when compared to DPPH (IC 50 77.5±0.4 μg/ml), SOD (IC 50 62.5±2.1 μg/ml), hydroxyl (IC 50 88.50±1.8 μg/ml) and NO (IC 50 67.5±2.2 μg/ml) scavenging activity. Conclusions: The methanolic extract of A. caudatus showed potent α -amylase inhibition and antioxidant activities.

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