Ginjupalli, Kishore and Armugam, Karthik and Shavi, Venkat Gopal and Bhat, Mahalingam and Udupa, N (2013) Development of RP-HPLC method for simultaneous estimation of lactic acid and glycolic acid. Der Pharma Chemica, 5 (4). pp. 335-334. ISSN 0975-413X

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Abstract

Quantification of the acidic monomers of biodegradable polymers plays an important role in understanding the degradation process. Aim of the present study was to develop a specific and accurate reversed phase high performance liquid chromatographic method (RP-HPLC) for the quantification of lactic acid (LA) and glycolic acid (GA) using conventional C18 column with ultra-violet detection. Mobile phase comprised of phosphate buffer (10 mM, pH 3.0) and acetonitrile at a ratio of 95:5 %v/v which resulted in the separation of LA and GA within 5 min run time. The stationary phase was conventional C18 column (250 x 4.6 mm, 5 μm). The detection was carried out at UV wavelength of 210 nm and the retention time was found to be 3.11 and 3.69 min for GA and LA respectively. The calibration curve was linear for the selected range with a coefficient of determination 0.9992 ± 0.0021 and 0.9975 ± 0.0015 for GA and LA respectively. The proposed method is sensitive, simple and cost effective compared to the previously reported methods utilizing the special columns. The developed method was validated and the recoveries of GA and LA were 98.51 ± 3.46% and 97.20 ± 2.84% respectively. The developed method can be very useful for the estimation of LA and GA during the degradation of polymers based on these acids.

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