Nuclear DNA fragmentation negatively affects zona binding competence of Y bearing mouse spermatozoa

Kumar, Dayanidhi and Upadhya, Dinesh and Uppangala, Shubhashree and Salian, Sujit Raj and Kalthur, Guruprasad and Adiga, Satish Kumar (2013) Nuclear DNA fragmentation negatively affects zona binding competence of Y bearing mouse spermatozoa. Journal of Assisted Reproduction and Genetics. pp. 1-5. ISSN 1573-7330

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Abstract

Purpose To investigate the influence of sperm DNA integrity on the zona binding ability of mouse spermatozoa in relation to their sex chromosomal constitution. Method(s) In this prospective experimental study, the sperm DNA fragmentation was induced by exposing testicular area of Swiss Albino mice (Mus musculus ) to different doses of γ-radiation (0, 2.5, 5.0 and 10.0 Gy). Sperm DNA fragmentation was quantified by single cell gel electrophoresis (comet assay). In vitro sperm zona binding assay was performed and the numbers of zona bound X and Y bearing spermatozoa were determined using fluorescence in situ hybridization (FISH). Result(s) The assessment of zona pellucida bound X and Ybearing spermatozoa using fluorescence in situ hybridization has revealed a unique binding pattern. The number of zona bound Y-spermatozoa declined significantly (P<0.01 to 0.0001) with increase in the DNA damage. The skewed binding pattern of X and Y-bearing sperm was strongly correlated with the extent of sperm DNA damage. Conclusion(s) The zona pellucida may have a role in preventing DNA damaged mouse sperm binding especially towards Y-bearing sperm. However, the exact mechanism behind this observation needs to be elucidated further

Item Type: Article
Uncontrolled Keywords: SpermDNAdamage; Spermzona binding; Functional competence
Subjects: Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Manipal
Date Deposited: 28 Oct 2013 04:05
Last Modified: 28 Oct 2013 04:05
URI: http://eprints.manipal.edu/id/eprint/137599

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