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Production of a Lipopeptide Biosurfactant by a Novel Bacillus sp. and Its Applicability to Enhanced Oil Recovery

Thivaharan, Varadavenkatesan and Ramachandra Murty, V (2013) Production of a Lipopeptide Biosurfactant by a Novel Bacillus sp. and Its Applicability to Enhanced Oil Recovery. ISRN Microbiology.

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Abstract

Biosurfactants are surface-active compounds derived fromvaried microbial sources including bacteria and fungi.They are secreted extracellularly and have a wide range of exciting properties for bioremediation purposes. They also have vast applications in the food and medicine industry.With an objective of isolating microorganisms for enhanced oil recovery (EOR) operations, the study involved screening of organisms from an oil-contaminated site. Morphological, biochemical, and 16S rRNA analysis of the most promising candidate revealed it to be Bacillus siamensis, which has been associated with biosurfactant production, for the first time. Initial fermentation studies using mineral salt medium supplemented with crude oil resulted in a maximum biosurfactant yield of 0.64 g/L and reduction of surface tension to 36.1mN/mat 96 h.Characterization studieswere done using thin layer chromatography and Fourier transform infrared spectroscopy. FTIR spectra indicated the presence of carbonyl groups, alkyl bonds, and C–H and N–H stretching vibrations, typical of peptides. The extracted biosurfactant was stable at extreme temperatures, pH, and salinity. Its applicability to EOR was further verified by conducting sand pack column studies that yielded up to 60% oil recovery.

Item Type: Article
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 07 Nov 2013 11:38
Last Modified: 16 Nov 2013 05:48
URI: http://eprints.manipal.edu/id/eprint/137670

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