Evaluation of antinociceptive and anti- 23(4): 651-661, Jul./Aug. 2013 inflammatory activities of extract and fractions of Eugenia jambolana root bark and isolation of phytoconstituents

Kodangala, Chandrashekar (2013) Evaluation of antinociceptive and anti- 23(4): 651-661, Jul./Aug. 2013 inflammatory activities of extract and fractions of Eugenia jambolana root bark and isolation of phytoconstituents. Revista Brasileira de Farmacognosia Brazilian Journal of Pharmacognosy, 23 (4). pp. 651-661. ISSN 0102-695X

[img] PDF
Brazilian journal of Pharmacognosy.pdf - Published Version
Restricted to Registered users only

Download (1MB) | Request a copy

Abstract

Eugenia jambolana Lam., Myrtaceae, is a widely distributed and traditionally well known plant in India. The root bark of the plant was extracted with ethanol and then successively fractionated into petroleum ether fraction, chloroform fraction, n-butanol fraction and methanol fraction. The extract and fractions of the plant material were evaluated for the antinociceptive activity by acetic acid-induced writhing test and formalin-induced nociception test, and anti-infl ammatory activity was screened by carrageenan-induced rat paw edema, cotton pellet induced granuloma formation and adjuvant induced arthritis in rat models. The test materials showed the antinociceptive and anti-infl ammatory effect in dose dependent manner and the petroleum ether fraction was found to be most potent among the test materials. At 400 mg/kg b.w., p.o. dose petroleum ether fraction signifi cantly inhibited 54.28% writhing response and 73.77% formalin induced nociception in mice. The fraction with same dose showed signifi cant 79.31% inhibition of carrageenan-induced rat paw edema, 57.78% anti-proliferative effect and 77.93% inhibition of adjuvant induced arthritis. The bioactive petroleum ether fraction was then subjected to column chromatography which led to isolate three compounds, namely, β-sitosterol, stigmasterol and lupeol. These compounds were characterized and identifi ed by IR, 1H NMR, 13C NMR and mass spectroscopy.

Item Type: Article
Uncontrolled Keywords: Adjuvant induced arthritis; anti inflammatory; antinociceptive; formalin induced nociception; Eugenia jambolana ;Myrtaceae.
Subjects: Pharmacy > MCOPS Manipal > Pharmacognosy
Depositing User: KMC Manipal
Date Deposited: 12 Nov 2013 04:11
Last Modified: 12 Nov 2013 04:11
URI: http://eprints.manipal.edu/id/eprint/137694

Actions (login required)

View Item View Item