Ovule culture of cotton with in vitro mutagenesis. Isolation of mutant lines for improved oil, lipid content and fatty acid compositions

Muthusamy, A (2011) Ovule culture of cotton with in vitro mutagenesis. Isolation of mutant lines for improved oil, lipid content and fatty acid compositions. Journal of the Swamy Botanical Club, 27. pp. 83-92.

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Abstract

Induced mutants altering the fatty acid composition have been extensively used in the breeding programs to develop new cultivars. The improvement of oil composition for C18 unsaturated fatty acids is one of the most important breeding objectives for the development of high-quality. Genetic selection for oil seed quality has been low intensity in nature and today an expanding array of new breeding goals for bio-oleochemical and technical uses. In addition, biotechnological innovations offer promising support to mutation breeding for the domestication or even construction of virtually new oil seed crops for application in both food and nonfood uses. The objective of the present study was aimed to analyze the induced changes in oil, lipid content and fatty acid composition in selected mutant lines of cotton using GR, EMS and SA through in vitro mutagenesis. The seeds of mutant lines 11, 7, 9, 5 and 2 showed significantly higher oil and lipid content than other mutants as well as control. These 5 mutants also haetwo fold increases in lipid contents. The Gas- chromatographic analysis of fatty acids revealed that, the mutant line 11 shows higher amounts of fatty acids followed by mutant lines 7, 9, 5 and 2. Among the 14 fatty acids quantified, highest amount of Cis- Linoleic acid was noted in terms of mg/g lipid individually between the mutant lines and totally among the mutant lines.

Item Type: Article
Uncontrolled Keywords: cotton: ovule culture: mutagenesis: mutant line; oil: lipid : fatty acid compositons.
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 27 Nov 2013 11:13
Last Modified: 27 Nov 2013 11:13
URI: http://eprints.manipal.edu/id/eprint/137839

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