MU Digital Repository
Logo

Thermochemotherapy: Synergism Between Hyperthermia and Liposomal Bleomycin in Mice Bearing Melanoma B16F1

Tiwari, Sandip B and Udupa, Venkatesh N and Rao, Satish BS and Devi, Uma P (2000) Thermochemotherapy: Synergism Between Hyperthermia and Liposomal Bleomycin in Mice Bearing Melanoma B16F1. Pharmacy and Pharmacology Communications, 6. pp. 19-23.

[img] PDF
Tiwari - PPC.pdf - Published Version
Restricted to Registered users only

Download (62kB) | Request a copy

Abstract

This study was aimed at enhancing the antitumour ef®cacy of bleomycin by encapsulating it in temperature-sensitive liposomes and using it in combination with localized hyperthermia of tumours for targeted delivery. Large unilammelar vesicles (LUV) made of synthetic lipids (disteroyl phosphatidylcholine and dipalmitoyl phosphatidylcholine) showing gel-to-liquid phase transition at 41�C, were used to encapsulate bleomycin. Comparison of LUV when incubated in saline at various temperatures revealed that maximum drug release (80%) occurred at 42�C compared with less than 5% release at 37�C. Better stability during storage was also observed with thermosensitive bleomycin liposomes. When administered intravenously to C57BL=6J mice bearing melanoma B16F1 tumour at 10 mg kg ÿ1 dose, liposomal bleomycin in combination with hyperthermia (43�C, 30 min or 1 h) exhibited improved anticancer activity as evident by the enhanced volume doubling time and growth delay compared with animals treated with an equivalent dose of f ee bleomycin with or without hyperthermia. The results suggest that hyperthermia in combination with bleomycin encapsulated inM temperature sensitive liposomes may be a useful targeted drug delivery system for more effective management of melanoma B16F1.

Item Type: Article
Subjects: Life Sciences > MLSC Manipal
Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Manipal
Date Deposited: 23 Dec 2013 10:02
Last Modified: 17 Dec 2015 09:19
URI: http://eprints.manipal.edu/id/eprint/138068

Actions (login required)

View Item View Item