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Improved sensitivity of microscopy for detection of Acid-Fast Bacilli in sputum by distilled water treated sodium hydroxide concentration method

*, Radhakrishna M and *, Veena Chennappa (2010) Improved sensitivity of microscopy for detection of Acid-Fast Bacilli in sputum by distilled water treated sodium hydroxide concentration method. Borneo Journal of Medical Sciences, 4 (1). pp. 23-25.

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Abstract

Tuberculosis is the major mycobacterial disease of the mankind in the world. The major objective of tuberculosis control programs is to identify and treat patients with infectious pulmonary tuberculosis. The conventional method of diagnosis of pulmonary tuberculosis relies on the bacteriological examination of sputum. The objective of the study was to see the increased sensitivity of AFB in direct microscopy by modified Petroff’s method using distilled water. Eight hundred early morning sputum samples were collected from patients of clinically diagnosed pulmonary tuberculosis. Smears were prepared from direct samples, after homogenization, after concentration method by Petroff’s method and by modified Petroff’s method adding distilled water and stained by Ziehl- Neelsen staining. Modified Petroff’s method by simple addition of equal quantity of distilled water increased the sensitivity of AFB smear result up to 45.5%. It was observed that centrifugation speed of 2000Xg is optimum for the maximum settlement of AFB by distilled water treated Petroff’s method of concentration

Item Type: Article
Uncontrolled Keywords: Acid-Fast Bacilli, Petroff’s method, Modified Petroff’s method, Sodium hydroxide method, Distilled water, Ziehl-Neelsen staining
Subjects: Medicine > KMC Mangalore > Microbiology
Depositing User: KMCMLR User
Date Deposited: 24 Dec 2013 11:19
Last Modified: 24 Dec 2013 11:19
URI: http://eprints.manipal.edu/id/eprint/138086

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