Antimicrobial efficacy of endodontic irrigants from Azadirachta indica: An in vitro study

Dutta, Arindam and *, Kundabala M (2013) Antimicrobial efficacy of endodontic irrigants from Azadirachta indica: An in vitro study. Acta Odontologica Scandinavica. pp. 1-5. ISSN 1502-3850

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Objective. This study analyzed the antimicrobial effect of five irrigants formulated from different parts of the tree Azadirachta indica (Neem) and compared with 2.5% sodium hypochlorite and 0.2% chlorhexidine gluconate through an agar diffusion test. Materials and methods. A clinical isolate of Candida albicans was innoculated on Sabourad Dextrose Agar and Enterococcus faecalis (ATCC 29212) on Sheep Blood Agar.Wells with 6 mm diameter were created in agar and 100 mL aliquiots of each irrigant were introduced to five different wells. After incubation, the largest uniform diameter of the inhibition zone was recorded. Results. The leaf extract of the tree andamixture of the seed-bark powder dissolved in dimethyl sulfoxide were active against both organisms. The other neembased irrigants, a leaf powder dissolved in dimethyl sulfoxide, aqueous bark decoction and neemoil, did not possess any antimicrobial efficacy. Sodium hypochlorite completely inhibited growth of C. albicans and the leaf extract had larger inhibition zones than chlorhexidine (p = 0.011) or the seed-bark irrigant (p = 0.008). Against E. faecalis, inhibition zones with chlorhexidine were the largest and differed significantly from sodium hypochlorite (p = 0.039), leaf extract (p = 0.008) and seed-bark irrigant (p = 0.011). Conclusions. Two neem irrigants displayed antimicrobial properties. The efficacy of the standard endodontic irrigants varied depending on the organisms tested. Clinical relevance: Neem-based endodontic irrigants may be formulated for clinical application.

Item Type: Article
Uncontrolled Keywords: Azadirachta indica, sodium hypochlorite, chlorhexidine gluconate, Candida albicans, Enterococcus faecalis
Subjects: Dentistry > MCODS Mangalore > Conservative Dentistry
Depositing User: KMCMLR User
Date Deposited: 30 Dec 2013 07:17
Last Modified: 30 Dec 2013 07:17

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