Protective Effect of Calendula Officinalis Linn. Flowers Against 3-nitropropionic acid Induced Experimental Huntington’s Disease in Rats

Bansal, Punit and Unnikrishnan, MK (2013) Protective Effect of Calendula Officinalis Linn. Flowers Against 3-nitropropionic acid Induced Experimental Huntington’s Disease in Rats. Drug and Chemical Toxicology, 36 (1). pp. 1-8. ISSN 1525-6014

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Abstract

Oxidative stress (OS) and nitric oxide mechanisms have been recently proposed in 3-nitropropionic acid (3-NP)-induced neurotoxicity. The compounds, having antioxidant, anti-inflammatory, and estrogenic effects, have been suggested for neuroprotection in different experimental models. Calendula officinalis Linn. flower extract (COE) is known for its potent antioxidant, anti-inflammatory, estrogenic, and neuroprotective activities. Hence, the present study was designed to evaluate the neuroprotective effect of COE on 3-NP-induced neurotoxicity in rats by observing behavioral changes, OS, and striatal damage in rat brain. Adult female Wistar rats were pretreated with vehicle or COE (100 and 200mg/kg) for 7 days, followed by cotreatment with 3-NP (15mg/kg, intraperitoneally) for the next 7 days. At the end of the treatment schedule, rats were evaluated for alterations in sensory motor functions and short-term memory. Animals were sacrificed and brain homogenates were used for the estimation of lipid peroxidation (LPO), glutathione, total thiols, glutathione S -transferase, catalase, and nitrite. A set of brain slices was used for the evaluation of neuronal damage in the striatal region of the brain. 3-NP caused significant alterations in animal behavior, oxidative defense system evidenced by raised levels of LPO and nitrite concentration, and depletion of antioxidant levels. It also produced a loss of neuronal cells in the striatal region. Treatment with COE significantly attenuated behavioral alterations, oxidative damage, and striatal neuronal loss in 3-NP-treated animals. The present study shows that COE is protective against 3-NP-induced neurotoxicity. The antioxidant,anti-inflammatory, and estrogenic properties of COE may be responsible for its neuroprotective action.

Item Type: Article
Uncontrolled Keywords: Excitotoxicity; Marigold; Oxidative stress; Neuroprotective; Nitrative stress; TTC
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 31 Dec 2013 04:54
Last Modified: 31 Dec 2013 04:54
URI: http://eprints.manipal.edu/id/eprint/138148

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