Design of Cultural Conditions for Enhancement of Alkaline Protease Production

Smrithi, S and Narayanan, K and Kamath, Venkatesh B (2013) Design of Cultural Conditions for Enhancement of Alkaline Protease Production. PHARMANEST - An International Journal of Advances in Pharmaceutical Sciences, 4 (6). pp. 1438-1446. ISSN 2231 - 0541

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Abstract

Among the various industrial enzymes studied, proteases are the most widely explored enzyme. They occupy the top position in terms of the amount of enzyme produced commercially. In the present study, soil samples collected from various locations around Manipal, Udupi District, Karnataka were screened for proteolytic activity. Casein agar medium was used as basal medium. The isolate showing highest activity during primary screening was further studied for proteolytic activity. Among the isolates screened, KWF - 2 obtained from drains of kitchen waste, showed significant proteolytic activity. Therefore, it was further studied by shake flask culture method. Protease yield was improved through cultural condition optimization. These conditions were optimized by one-factor-at-a- time method. The protease was found to be an alkaline protease. Due to the promising result, further studies on characterization of the enzyme are being carried out.

Item Type: Article
Uncontrolled Keywords: Screening; Protease; Optimization
Subjects: Life Sciences > MLSC Manipal
Pharmacy > MCOPS Manipal > Pharmaceutical Biotechnology
Depositing User: KMC Manipal
Date Deposited: 01 Jan 2014 04:22
Last Modified: 01 Jan 2014 04:22
URI: http://eprints.manipal.edu/id/eprint/138165

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