Koteshwara, KB and Naha, Anup and Sunil, P and Sai, Kiran (2013) Method Development and Validation of Glibenclamide in Tablet Dosage Form by using RP-HPLC. Journal Of Harmonized Research in Pharmacy, 2 (4). pp. 226-230. ISSN 2321 – 0958

PDF Article_JOHR_Dec 2013.pdf - Published Version Restricted to Registered users only Download (114kB) | Request a copy

Abstract

A simple, precise, reliable, rapid and reproducible reversed phase–high-performance liquid chromatography method was developed and validated for the estimation of glibenclamide. Chromatographic separation was achieved isocratical ly with Grace Vydac C 18 column (250 × 4.6 mm, 5 μ ) and Acetonitrile:20 mM ammonium acetate buffer, pH 4.5 (50:50) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at 300 nm. Parameters such as linearity, precision, accuracy, robustness recovery are studied as per IC H guidelines. The retention time for Glibenclamide was found to be 15 min. Linearity for glibenclamidewas in the range of10–70 μ g/ml and mean recoveries obtained for glibenclamide was9 9.22±1.07% and relative standard deviation (RSD) was0.90. The correlation coefficients for all components were close to 1. Developed method was found to be accurate, precise, selective and rapid for estimation of glibenclamide.

Actions (login required)

View Item