Carbapenem resistance in clinically significant Non fermenting gram negative bacilli

Bhat, Sevitha and Sharma, Renu and *, Zenith Euphemia (2013) Carbapenem resistance in clinically significant Non fermenting gram negative bacilli. Journal of Evolution of Medical and Dental Sciences, 2 (47). pp. 9131-9134.

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Abstract

BACKGROUND/OBJECTIVES: The growing increase in the rates of antibiotic resistance is a major cause for concern in both non-fermenting bacilli .β-lactams have been the mainstay of treatment for serious infections, and the most active of these are the carbapenems. Acquired metallo-β-lactamases (MBL) have recently emerged as one of the most worrisome resistance mechanisms owing to their capacity to hydrolyze all β-lactams, including carbapenems. We have undertaken this study to ascertain the prevalence of MBL-producing non-fermenting bacilli. MATERIALS AND METHODS: The study included 93 isolates of NFGNB from Blood, endotracheal aspirates, sputum, urine, urinary catheter tip & pus between January 2012- January 2013. Antibiotic susceptibility to antibiotics was done by Modified Kirby Bauer method. All the isolates with a zone size ≤19mm for imipenem were included in our study to detect the presence of MBL by Imipenem-EDTA double-disc synergy test (DDST). Imipenem resistant, non MBL isolates were tested by Modified Hodge test & Amp C β lactamases. RESULTS: Of the 93 imipenem resistant NFGNB, 27(29.03%) were Pseudomonas spp. ,66(70.96%) were Acinetobacter spp.Among them, 10(37.03%) Pseudomonas spp. and seven (10.60%) Acinetobacter spp. were MBL positive by Imipenem EDTA double-disc synergy test (DDST). Five (8.40%) Acinetobacter spp. were Amp C positive and one (5.88%)strain of Pseudomonas showed positive Modified hodge test. CONCLUSION: MBL production is an important mechanism of carbapenem resistance among Pseudomonas spp. but not among Acinetobacter spp. Carbapenemases other than MBL may be responsible for carbapenem resistance in NFGNB.

Item Type: Article
Uncontrolled Keywords: Non fermenting gram negative bacilli, imipenem, metallo-β-lactamases
Subjects: Medicine > KMC Mangalore > Microbiology
Depositing User: KMCMLR User
Date Deposited: 09 Jan 2014 11:41
Last Modified: 09 Jan 2014 11:41
URI: http://eprints.manipal.edu/id/eprint/138305

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