Mitochondrial DNA Variation Analysis in Cervical Cancer

Kabekkodu, Shama Prasada and Bhat, Samatha and Mascarenhas, Roshan and Mallya, Sandeep and Bhat, Manoj and Pandey, Deeksha and Kushtagi, Pralhad and Gopinath, PM and Satyamoorthy, K (2013) Mitochondrial DNA Variation Analysis in Cervical Cancer. Mitochondrion.

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Abstract

This study was undertaken to investigate the mitochondrial DNA (mtDNA) variation in non-malignant and malignant cervical tissue samples. We have identified 229 and 739 variations non-malignant and malignant tissues respectively distributed over 321 locations in the D-loop (50 in non-malignant and 166 in malignant; 216 variations), coding region (139 in non-malignant and 455 in malignant; 594 variations) tRNA and rRNA genes (39 in non-malignant and 119 in malignant; 158 variations). Besides, 77 novel and 34 various other disease associated variations were identified in non-malignant and malignant samples. A total of 236 tumor specific variations in 201 locations representing 30.1% in D-loop, 59.3% in coding regions and 10.6% in RNA genes were also identified. Our study shows that D loop (in 67 locations) is highly altered followed by ND5 (35 locations) region. Moreover, mtDNA alterations were significantly higher in malignant samples by two tailed Fisher's exact test (P ≤ 0.05) with decreased mtDNA copy numbers. Bioinformatic analysis of 59 non-synonymous changes predicted several variations as damaging leading to decreased stability of the proteins. Taken together, mtDNA is highly altered in cervical cancer and functional studies are needed to be investigated to understand the consequence of these variations in cervical carcinogenesis and their potential application as biomarkers.

Item Type: Article
Uncontrolled Keywords: Cervical cancer; mtDNA; HPV; Copy number; Haplogroup; D loop and coding region
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Manipal
Date Deposited: 15 Jan 2014 07:39
Last Modified: 04 Nov 2016 14:11
URI: http://eprints.manipal.edu/id/eprint/138360

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