Correlation of Sputum Culture with Serology Against Mycoplasma pneumoniae in Patients with Bronchial Asthma

Sahoo, Rameshchandra and Acharya, Vishak K. and Shenoy, Shalini and *, Anand R and Reddy, Rama Keshava (2007) Correlation of Sputum Culture with Serology Against Mycoplasma pneumoniae in Patients with Bronchial Asthma. The Indian Journal of Chest Diseases & Allied Sciences, 49. pp. 209-212.

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Objectives. Mycoplasma pneumoniae is implicated in acute exacerbations of bronchial asthma and is also a factor in chronicity of asthma. Methods. We attempted to isolate M. pneumoniae in sputum specimen samples by culture technique and also by serological estimation of immunoglobulin (lgM, IgG) antibodies against M. pneumoniae by enzyme linked immunosorbent assay (ELISA) in 100 patients with bronchial asthma and 50 subjects without asthma who served as controls. Results. Mycoplasma pneumoniae was isolated by sputum culture in 17% of patients with asthma and in none of the control subjects. Immunogloublin (lgG/lgM) antibodies against M. pneumoniae were found in 37% of patients with asthma and 6% of control subjects. On correlating culture with serology, it was found that 15% patients were both culture and seropositive, 2% patients were culture positive but seronegative, 22% patients were culture negative but seropositive while 61% patients were both culture and seronegatives. The sensitivity and specificity of culture were 73.5% and 88.2%, respectively. For the serological method, sensitivity and specificity were 88.2% and 73.5%, respectively. Conclusions. Use of a combination of sputum culture and serological methods are reliable investigational tools for the diagnosis of M. pneumoniae infection. The significant association observed between M. pneumoniae and bronchial asthma justifies the inclusion of antibiotics, such as the macrolides, in treating these patients.

Item Type: Article
Uncontrolled Keywords: Mycoplasma pneumoniae, Bronchial asthma, Sputum culture, Serology
Subjects: Medicine > KMC Mangalore > Microbiology
Medicine > KMC Mangalore > Medicine
Depositing User: KMCMLR User
Date Deposited: 28 Jan 2014 07:37
Last Modified: 28 Jan 2014 07:37

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