Micro-Raman Spectroscopy for Optical Pathology of Oral Squamous Cell Carcinoma

Murali Krishna, C and Sockalingum, GD and Kurien, Jacob and Rao, Lakshmi and Venteo, L and Pluot, M and Manfait, M and Kartha, VB (2004) Micro-Raman Spectroscopy for Optical Pathology of Oral Squamous Cell Carcinoma. Applied Spectroscopy, 58 (9). pp. 1128-1135.

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Micro-Raman spectra of formalin-fixed oral squamous normal and carcinoma tissues, stored at room temperature for 2 months, have been recorded. Spectra were recorded both in the epithelial and subepithelial regions of the tissues. No noticeable spectral contamination due to formalin was observed. Very significant differences between spectra of normal epithelial and malignant epithelial samples were found. No such differences in spectra of subepithelial malignant and subepithelial normal samples could be observed. This study shows that spectra from the epithelial region changes drastically because of malignancy-induced biochemical changes in this region. Major differences between normal and malignant spectra seem to arise from the protein composition, conformational/structural changes, and possible increase in protein content in malignant epithelia. The differences between normal epithelial and subepithelial spectra, as expected, arise mainly from the collagen in subepithelial tissue. Principal component analysis of the combined sets of spectra—epithelial and subepithelial, normal and malignant — showed that very good discrimination can be achieved by Raman microspectroscopy. This study thus validates the suitability of formalin- fixed tissues for optical pathology in oral malignancy. Index Headings: Micro-Raman spectroscopy; Oral cancer; Optical pathology; Formalin-fixed tissues.

Item Type: Article
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Pathology
Medicine > KMC Manipal > Surgical Oncology
Depositing User: KMC Manipal
Date Deposited: 30 Sep 2011 04:43
Last Modified: 30 Sep 2011 04:43
URI: http://eprints.manipal.edu/id/eprint/1387

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