Estimation of glycated hemoglobin by 2,6-dimethylphenol: Sulphuric acid conventional method

Mallya, Madhukar H (2001) Estimation of glycated hemoglobin by 2,6-dimethylphenol: Sulphuric acid conventional method. Indian Journal of Clinical Biochemistry, 16 (1). pp. 37-41.

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Glycated hemoglobin levels in hemolysate of normal and diabetic patients were determined by the 2,6-dimnthylphenol:67.5% sulphudc acid conventional method and the values were 0.3930.025 and 0.5910.21 moles of hydroxymethylfurfural(HMF)/mole of globin, respectively. The mean increase in glyceted hemoglobin values in diabetics (1.8 fold) was highly significant (p<0.001). A good correlation (r--0.95) was found the glycated hemoglobin values obtained by this method and the phenol:sulphudc acid method. The values obtained by former method were about times the values by the phenoi:sulphuric acid method. This study indicates that conventional 2,6- dimethylphenoh57.5% suiphuric acid method is more sensitive for the estimation of glycetsd hemoglobin than any other method based on the same principle, it is less time consuming, reliable and hence can be employed for the routine laboretoW estimation of glyceted hemoglobin for the assessment of glycemic control.

Item Type: Article
Uncontrolled Keywords: Diabetes Mellitus; Estimation of glycat~d proteins; Glycated hemoglobin.
Subjects: Medicine > KMC Manipal > Biochemistry
Depositing User: KMC Manipal
Date Deposited: 10 Feb 2014 05:03
Last Modified: 10 Feb 2014 05:03

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