Development and validation of RP-HPLC method with ultraviolet detection for estimation of montelukast in rabbit plasma: Application to preclinical pharmacokinetics

Ranjan, Om Prakash and Nayak, Usha Y and Reddy, Meka Sreenivasa and Dengale, Swapnil J and Musmade, Prashant B and Udupa, Nayanabhirama (2013) Development and validation of RP-HPLC method with ultraviolet detection for estimation of montelukast in rabbit plasma: Application to preclinical pharmacokinetics. Journal of Young Pharmacists, 5. pp. 133-138. ISSN 0975-1483

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Abstract

Objective: To develop a liquid–liquid extraction based reverse phase liquid chromatography method for estimation of montelukast in rabbit plasma. Methods: Chromatographic separation was carried out using Phenomenex Luna C18 column (250mm� 4.6mm� 5 mm) with mobile phase composed of ammoniumacetate buffer (20Mm), pH 5.5 and acetonitrile in 20:80, v/v ratio. The analytewasmonitored withUV detector at 345nm. The developedmethod was validated with respect to linearity, accuracy, precision, specificity and stability. Results: The peak area ratio of montelukast (MKS) to that of internal standard was used for the quantification of samples. Calibration curves were linear in the concentration range of 20e2000 ng mL�1. The LOD and LLOQ of present method were found out to be 10 ng mL�1 and 20 ng mL�1 respectively. The intra-day and inter-day %CV values for MKS were below 6.06% and 8.43%. Intra-day and inter-day accuracies were within 95.81% and 110.90%, respectively. Extraction recoveries of drug from rabbit plasma were >66.47%. Conclusion: A simple, alternative, reproducible and sensitive HPLC-UV method was developed for MKS that can be used in preclinical pharmacokinetics.

Item Type: Article
Uncontrolled Keywords: Montelukast; Rabbit plasma; HPLC; UV detection; Pharmacokinetics.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Quality Assurance
Pharmacy > MCOPS Manipal > Pharmaceutics
Pharmacy > MCOPS Manipal > Pharmacy Management
Depositing User: KMC Manipal
Date Deposited: 14 Feb 2014 04:31
Last Modified: 14 Feb 2014 04:31
URI: http://eprints.manipal.edu/id/eprint/138837

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