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A comprehensive comparison of PCR based assay versus microscopy & culture in the diagnosis of tuberculosis in different clinical specimens

*, Arvind Natarajan and *, Pavan Chand and Baliga, Shrikala and *, Bharati B and Shenoy, Shalini and Saralaya, Vishwas (2014) A comprehensive comparison of PCR based assay versus microscopy & culture in the diagnosis of tuberculosis in different clinical specimens. International Journal of Biological & Medical Research, 5 (1). pp. 3763-3768. ISSN 0976:6685.

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Abstract

Background: The laboratory diagnosis of tuberculosis ranges from simple microscopy, culture to complex molecular techniques. Rapid diagnosis is important since conventional techniques have limitations. Aims: To evaluate the efficacy of PCR using genus specific and species specific primers versus microscopy and culture in the diagnosis of tuberculosis in different clinical samples. Methods: A total of 125 clinical samples were processed for the detection of Mycobacterium tuberculosis by smear, culture & PCR in the diagnosis of tuberculosis. Results: The overall sensitivity, specificity, PPV and NPV of PCR were 75 %, 88%, 62.5% and 93.42% respectively. PCR done on non tuberculous patients were negative thus showing the specificity of 100%. PCR sensitivity in pulmonary & extra pulmonary clinical samples were 83.33% and 71.42% respectively a relatively higher value than other tests. Out of eight smear positive-culture positive (SP-CP) & twelve smear negative –culture positive (SNCP) specimens, PCR detected 87.50 % & 66.66% respectively. Conclusions: This study showed that PCR can serve as a useful complement to clinical diagnosis. PCR can be useful in detecting the cases in extra pulmonary specimens which may be missed by smear &\ or culture. The PCR based assay cannot be used reliably alone as a sole diagnostic tool in the diagnosis of tuberculosis, it has to be performed in conjunction with microscopy & culture methods and interpreted with the clinical settings.

Item Type: Article
Uncontrolled Keywords: Comparison of microscopy Culture & PCR in different Clinical specimens: Tuberculosis
Subjects: Medicine > KMC Mangalore > Microbiology
Depositing User: KMCMLR User
Date Deposited: 11 Mar 2014 09:46
Last Modified: 11 Mar 2014 09:46
URI: http://eprints.manipal.edu/id/eprint/139081

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