Analytical method development and validation of simultaneous estimation of amlodipine and atorvastatin by RP-UPLC

Waghmare, AN and Muddukrishna, BS and Vasantharaju, SG (2014) Analytical method development and validation of simultaneous estimation of amlodipine and atorvastatin by RP-UPLC. Mintage Journal of Pharmaceutical & Medical Sciences, 3 (2). pp. 22-25.

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Abstract

Objective: To develop and validate simple, sensitive, robust, rapid and specific isocratic RP-UPLC method for simultaneous estimation of Amlodipine and Atorvastatin in tablet dosage form. Methods: The developed method consisting the mobile phase of acetonitrile and 0.02 M Potassium dihydrogen phosphate (55:45) with isocratic programming, BEH C18 (100mm×2.1mm, 1.7μm) column as stationary phase with a flow rate of 0.3 mL/minute. Results and discussion: Proposed method was found to be linear for Amlodipine and Atorvastatin in the concentration range of 0.5 to 40.0 μg/mL with r2 of 0.9999 for Amlodipine and 0.9997 for Atorvastatin respectively. Precision study showed that the percentage relative standard deviation was within the range of acceptable limits, and the mean recovery was found to be 100.79 % for assay of Amlodipine and 99.87% for Atorvastatin in tablet dosage form .The LOD and LOQ of Amlodipine and Atorvastatin were found to be 0.062 and 0.078μg/ml and 0.020 and 0.026 μg/mL.

Item Type: Article
Uncontrolled Keywords: Amlodipine; Atorvastatin&UPLC method.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Quality Assurance
Depositing User: KMC Manipal
Date Deposited: 20 Mar 2014 06:40
Last Modified: 20 Mar 2014 06:40
URI: http://eprints.manipal.edu/id/eprint/139166

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