Leucoagglutination in vitro

Nayak, Deepak M and Patil, Asha and Dhakal, Rachana (2013) Leucoagglutination in vitro. Journal of Evolution of Medical and Dental Sciences, 2 (5). pp. 467-472. ISSN 2278-4802

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Abstract

Phenomenon such as cold agglutination of red blood cells and platelets are well recognized and extensively studied. Nevertheless, aggregation of leukocytes in vitro is a rare phenomenon and may result in pseudoleukopenia. The exact mechanism for this phenomenon has not been elucidated. We report one such rare case where a male patient with HIV presented with leucopenia. The analysis of ethylene diamine tetraacetic acid (EDTA) anticoagulated blood was done on Coulter LH 750 hematology analyzer. The Volume Conductivity Scatter (VCS) parameters and histogram generated were inconclusive .The peripheral smear revealed small aggregates of leucocytes. The leucoaggregates were reversed on incubating the blood sample at 37 degree Celsius for 30 minutes. This suggests the role of a temperature dependent antibody causing clumping of leucocytes; possibly in conjunction with EDTA.

Item Type: Article
Uncontrolled Keywords: leucoaggregates; EDTA; antibody; leucocytes.
Subjects: Allied Health > MCOAHS Manipal > Medical Laboratory Tech
Medicine > MMMC Manipal > Pathology
Medicine > KMC Manipal > Pathology
Depositing User: KMC Manipal
Date Deposited: 22 Mar 2014 04:26
Last Modified: 22 Mar 2014 04:26
URI: http://eprints.manipal.edu/id/eprint/139178

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