Pilot study Of erythrocyte acetlycholinesterase, plasma phosphodiesterase and protein thiols in diabetic patients

Thomas, Ann T and Ramanan, Harini and Datta, Abhinandya and Prabhu, Krishnananda and Rao, Anjali and Thakur, Rajesh Kumar and Gyawali, Prabin and Mukherjee, Madhurima (2010) Pilot study Of erythrocyte acetlycholinesterase, plasma phosphodiesterase and protein thiols in diabetic patients. In: ACBICON 2010, 13-15 Dec 2010, Mumbai, India. (Submitted)

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Abstract

INTRODUCTION: Numerous studies have demonstrated that oxidative stress, mediated mainly by hyperglycemia-induced generation of free radicals, contributes to the development and progression of diabetes. Changes in the activity of acetylcholinesterase (AChE) have been reported in diabetes mellitus and was significantly lower in type 2 diabetic patients than in normal controls and in vitro insulin treatment restored this activity to normal levels. The –SH group (reduced thiols) bound to proteins (protein thiols) play a major role in maintaining the antioxidant status of the body. Such thiols have been studied in different disease conditions and found to be decreased compared to healthy control samples. Phosphodiesterase plays an important role in the antilipolytic action of insulin and when reduced will lead to a reduced ability of insulin to antagonize lipolysis. MATERIALS AND METHODS: Institutional Ethics Committee gave permission to undertake this study. After obtaining prior consent, blood (2 ml) was taken using aseptic precautions from diabetic patients (n = 30) and age and sex matched healthy controls (n = 20) in heparinised vacutainers. RESULTS: There was a significant difference in the levels of protein thiols (p=116.30 ), acetylcholinesterase (median=66.42) and phoshodiesterease( median=17.97 ) levels in the diabetic patients when compared to healthy controls. CONCLUSION: There may be a role for these markers in pathophysiology of diabetes mellitus.

Item Type: Conference or Workshop Item (Paper)
Subjects: Medicine > KMC Manipal > Biochemistry
Depositing User: KMC Manipal
Date Deposited: 01 Apr 2014 04:15
Last Modified: 01 Apr 2014 04:15
URI: http://eprints.manipal.edu/id/eprint/139262

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