Evaluation of Oxidative Stress and Antioxidants in Serum and Follicular Fluid of Women Undergoing Invitro Fertilization

Kumar, Pratap and Adiga, Satish Kumar (2014) Evaluation of Oxidative Stress and Antioxidants in Serum and Follicular Fluid of Women Undergoing Invitro Fertilization. International Journal of Basic and Life Sciences, 2 (2). pp. 1-10. ISSN 2320 - 513X

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Abstract

Objective:To evaluate the role of oxidative stress, antioxidants and estrogen in serum and follicular fluid (FF) of women undergoing IVF, and study their association with fertilization potential, maturity grade of oocytes, embryo quality, type of stimulation, age of patients and duration of subfertility.Methods:FF collected by trans-vaginal ultrasound guided oocyte rupture and intravenous blood from 28 women (20-40 years) undergoing IVF/ICSI was analyzed for FRAP, Nitrite, LDH, Protein carbonyls and estradiol.Their association with various IVF outcome parameters was studied.Result(s):Positive correlation was seen between Ferric Reducing Anti oxidant power (FRAP) and fertilization potential of oocytes (r=0.396, P=0.037). FRAP was comparatively high in serum (P= 0.048) ,whereas nitrite, protein carbonyls, estradiol were high in FF (P =0.0001 for all). FRAP in serum and FFshowed significant positive correlation. FRAP correlated with nitrite and LDH in FF. Long protocol was associated with increased fertilization potential of oocytes (r=0.369,P =0.05) and high protein carbonyls in FF. No effect was observed for OS markers on oocyte maturity or embryo quality. Duration of Subfertility correlated positively with age (P =0.01) but negatively with LDH in FF.Conclusion: FRAP in FF is associated with increased fertilization potential of oocytes. Low FRAP in FF and significant correlation of FRAP with nitrite and LDH in FF shows that antioxidants are used to overcome the oxidative damage. Long protocol is associated with increase in fertilization potential and protein carbonyls in FF. Age of patients correlates with duration of subfertility.

Item Type: Article
Uncontrolled Keywords: FRAP; Nitrite; LDH; Protein Carbonyls; Fertilization Potential of Oocytes
Subjects: Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Manipal
Date Deposited: 03 Apr 2014 04:09
Last Modified: 03 Apr 2014 04:09
URI: http://eprints.manipal.edu/id/eprint/139279

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