Evaluation of plasma phosphodiesteaseactivity in oral cancer

Prabhu, Krishnananda and Naik, Divya and Ray, Satadru M and Vadiraj, * and Rao, Anjali (2010) Evaluation of plasma phosphodiesteaseactivity in oral cancer. In: IBSC conference, 29 Dec - 01 Dec 2010, Penang, Malaysia. (Submitted)

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Introduction:Oral squamous cell carcinoma (OSCC) is one of the most common malignancies recognized nowadays, and represents a public health problem. Its early detection is the better alternative to provide a good quality of life for the patients. During the last years, several studies have identified potential biomarkers of OSCC progression and prognosis (1). The Phosphodiesterases (PDEs) are responsible for the hydrolysis of the second messengers with a fundamental role in the transduction of the intracellular signals. Variations in PDE activity have been correlated to different pathological mechanisms, such as cellular differentiation, apoptosis, and tumor invasivity(2, 3).PDEs are also known to play a role in tumor growth by influencing angiogenesis (4, 5). Objective: To estimate plasma PDE levels in oral cancer patients and compare it with age/sex matched healthy controls. Also to compare PDE levels in cancer cases after completion of radiotherapy with respective pre treatment levels. Materials and methods: Institutional Ethics Committee gave us the permission conduct this study. After obtaining consent from biopsy proven patients (n= 39), 2ml of blood was collected in EDTA Vacutainers before onset of any definitive treatment and again after the course of radiotherapy. From controls (n=27) blood was collected only once. Plasma phosphodiesterasewas estimated by colorimetric method in both the groups. Results and Conclusion:There was a significant increase in PDE values in cases as compared to controls (p < 0.0001). Amongst the cases there was a significant decrease in post radiotherapy values as compared to respective pre radiotherapy levels (p < 0.0001). This indicates that there is a role for phophodiesterase in pathophysiology of cancer. Inhibitors specific for individual PDE isoenzymes may selectively restore normal intracellular signaling, providing antitumor therapy with reduced adverse effects.

Item Type: Conference or Workshop Item (Paper)
Subjects: Medicine > KMC Manipal > Biochemistry
Depositing User: KMC Manipal
Date Deposited: 03 Apr 2014 04:49
Last Modified: 03 Apr 2014 04:49
URI: http://eprints.manipal.edu/id/eprint/139288

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