Can cooling of blood sample be an effective alternative for sodium fluoride as a preservative for blood glucose samples?

Gyawali, Prabin and Mukherjee, Madhurima and Prabhu, Krishnananda and Rao, Anjali and Sharma, Stuti and Mohan, Sheetal and Thakur, Rajesh Kumar and Mishra, Manoj (2008) Can cooling of blood sample be an effective alternative for sodium fluoride as a preservative for blood glucose samples? In: International Conference on"Free Radicals and Natural Products in Health” , 14-16 Feb 2008, Jaipur, India. (Submitted)

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Abstract

Introduction: A decline in glucose values by 10% occurred when they were stored for 48 h before analysis and this decline persisted in samples kept at room temperature, but was abolished by storage at 4 °C. Even with the use of fluoride it takes approximately three hours for these glycolytic inhibitors to become fully effective and stabilize glucose levels. Hypothesis: If we avoid sodium fluoride as a preservative then the same samples can be used for other assays in cases when sample size must be restricted. Materials and methods: Six samples in duplicate one with fluoride and other without fluoride are taken from diabetic patients attending our out patient department. The samples with fluoride are kept at room temperature and samples without are kept at 4 degrees. In both groups blood was centrifuged and kept without separating the seum were processed every hour for three hours so as to find the difference the glucose values. Results: Fluorinated sample showed 102.3 mg% at zero and 99.66 mg% at three hrs respectively(decrease of 3 mg) and in non fluorinated refrigerated samples showed 100 and 87 respectivly(decrease of 13 mg).However the decrease was not statistically significant. Conclusion: Our results show that effective cooling of blood samples immediately after withdrawal can be an effective preservative than the fluoride and also we feel it should be recommended as it avoids taking two different samples if the patient wants some other tests and in cases where samples should be restricted.

Item Type: Conference or Workshop Item (Paper)
Subjects: Medicine > KMC Manipal > Biochemistry
Depositing User: KMC Manipal
Date Deposited: 05 Apr 2014 04:07
Last Modified: 05 Apr 2014 04:07
URI: http://eprints.manipal.edu/id/eprint/139311

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