Protective effect of Cissus quadrangularis Linn. on diabetes induced delayed fetal skeletal ossification

Rao, Srinivasa Sirasanagandla and Pai, Sreedhara Ranganath K and Bhat, Kumar MR (2014) Protective effect of Cissus quadrangularis Linn. on diabetes induced delayed fetal skeletal ossification. Journal of Ayurveda & Integrative Medicine, 5 (1). pp. 25-32.

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Abstract

Background: Delayed fetal skeletal ossification is one of the known complications of maternal diabetes. Objective: The present study was designed to evaluate the protective role of petroleum ether extract of Cissus quadrangularis (PECQ) on diabetes-induced delayed fetal skeletal ossifi cation. Materials and Methods: Female Wistar rats were rendered diabetic with streptozotocin (STZ, 40 mg/kg, intraperitonial) before mating. After confi rmation of pregnancy, the pregnant rats were divided into three groups: normal control group, diabetic control group, and diabeticCQ group. The diabeticCQ group pregnant rats were treated with PECQ (500 mg/kg body weight) throughout their gestation period. Immediately after delivery, pups were collected from all three groups and processed for alizarin red S–alcian blue staining in order to examine the pattern of skeletal ossifi cation. Results: Fewer ossifi cation centers and decreased extent of ossifi cation of forelimb and hindlimb bones were observed in the neonatal pups of diabetic control group as compared to those in the normal control group. PECQ pretreatment signifi cantly restored the ossifi cation centers and improved the extent of ossifi cation of forelimb and hindlimb bones in the neonatal pups of diabeticCQ group as compared to those in the diabetic control group. Conclusions: The results suggested that PECQ treatment is effective against diabetes-induced delayed fetal skeletal ossifi cation. However, further studies on the isolation and characterization of active constituents of PECQ, which can cross the placental barrier and are responsible for the bone anabolic activity are warranted.

Item Type: Article
Uncontrolled Keywords: Alizarin red-alcian blue; Cissus quadrangularis; maternal diabetes; ossifi cation centers; skeletal ossifi cation; streptozotocin.
Subjects: Medicine > KMC Manipal > Anatomy
Medicine > MMMC Manipal > Anatomy
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 19 Apr 2014 04:21
Last Modified: 19 Apr 2014 04:21
URI: http://eprints.manipal.edu/id/eprint/139403

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