Upregulation of TFAM and mitochondria copy number in human lymphoblastoid cells

Chakrabarty, Sanjiban and D'Souza, Reena Reshma and Kabekkodu, Shama Prasada and Gopinath, Puthiya M and Satyamoorthy, K (2014) Upregulation of TFAM and mitochondria copy number in human lymphoblastoid cells. Mitochondrion, 15. pp. 52-58.

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Abstract

Mitochondria are central to several physiological and pathological conditions in humans. In the present study,we performed copy number analysis of nuclear encodedmitochondrial genes, in peripheral bloodmononuclear cells (PBMCs) and its representative lymphoblastoid cells (LCLs).We have observed hyper diploid copies of mitochondrial transcription factor A (TFAM) gene in the LCLs along with increased mtDNA copy number, mitochondrial mass, intracellular ROS and mitochondrial membrane potential, suggesting elevated mitochondrial biogenesis in LCLs. Gene expression analysis confirmed TFAM over-expression in LCLs when compared to PBMC. Based on our observation, we suggest that increased copy number of TFAM gene upregulates its expression, increases mtDNA copy numbers and protects it from oxidative stress induced damage in the transformed LCLs.

Item Type: Article
Uncontrolled Keywords: Mitochondria, Lymphoblastoid cell lines, Copy number, TFAM
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 15 May 2014 04:13
Last Modified: 15 May 2014 04:13
URI: http://eprints.manipal.edu/id/eprint/139530

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