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Pulsed Laser Induced Simultaneous Fluorescence and Photoacoustic Spectroscopic Studies of Flavin adenine Dinucleotide (FAD) in Water

Kamath, Sudha D and Mahato, KK (2012) Pulsed Laser Induced Simultaneous Fluorescence and Photoacoustic Spectroscopic Studies of Flavin adenine Dinucleotide (FAD) in Water. In: Electronic Design & Signal Processing. Narosa Publishing House Pvt. Ltd., pp. 112-116. ISBN 978-81-8487-160-9

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Abstract

Flavin-adenine dinucleotide (FAD) is an important coenzyme of various flavoproteins in our body. It displays highly heterogeneous fluorescence intensity decay in solution. In the present study, we have recorded the fluorescence and photoacoustic spectra at different concentrations of FAD simultaneously, showing how the radiative and non-radiative relaxation pathways differ as a function of concentration. In this paper, an Experimental system for measuring simultaneously photoacoustic (PA) and fluorescence signals setup is described. The simultaneous measurement laser induced fluorescence and photoacoustic signals provide a suitable method for the study of different quenching phenomena occurring in fluorescent systems. This paper reports, FAD solvation dynamics in water using fluorescence and photoacoustic spectra recorded simultaneously by photoacoustic and fluorescence signals as functions of concentration indicate that quantum yield is maximum at low concentrations. Also, the energy lost in the fluorescence path of FAD, due to different quenching phenomena like self-quenching, RET, solvation relaxation etc is clearly seen from the photoacoustic signal intensity which increases as the fluorescence intensity decreases.

Item Type: Book Section
Uncontrolled Keywords: Flavin Adenine dinucleotide, Fluorescence, quantum yield, Photo acoustic spectroscopy
Subjects: Engineering > MIT Manipal > Physics
Depositing User: MIT Library
Date Deposited: 02 Jun 2014 11:22
Last Modified: 02 Jun 2014 11:22
URI: http://eprints.manipal.edu/id/eprint/139626

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