Ovarian tissue vitrification is more efficient than slow freezing in protecting oocyte and granulosa cell DNA integrity

Mathias, Freya Jothsna and D’souza, Fiona and Uppangala, Shubhashree and Salian, Sujith R and Kalthur, Guruprasad and Adiga, Satish Kumar (2014) Ovarian tissue vitrification is more efficient than slow freezing in protecting oocyte and granulosa cell DNA integrity. Systems Biology in Reproductive Medicine. pp. 1-6. ISSN 1939-6368

[img] PDF
Mathias et al_SBRM_2014[1].pdf - Published Version
Restricted to Registered users only

Download (208kB) | Request a copy

Abstract

Ovarian tissue cryopreservation is the primary treatment modality currently available to women at risk of losing their ovarian function due to cytotoxic therapy. However, the impact of these techniques on the oocyte DNA integrity is not elucidated. Here we have investigated the effect of vitrification and conventional slow freezing of eight week old Swiss albino mouse ovarian tissues on the oocyte and granulosa cell DNA integrity using the comet assay. The intracellular levels of reactive oxygen species in oocytes was measured by 20,70-dichlorodihydrofluorescein diacetate fluorescence. The cryopreservation of ovarian tissue by the slow freezing technique resulted in a significantly higher level of DNA fragmentation in oocytes in comparison to vitrification (p50.05) whereas DNA fragmentation in granulosa cells was significantly higher than the control (p50.01). Further, reactive oxygen species were significantly elevated in oocytes derived from slow freezing when compared to vitrification (p50.05). Therefore, we conclude that the ovarian tissue slow freeze-thawing makes the oocyte and granulosa cells more vulnerable to DNA damage whereas vitrification appears to be a safer method than slow freezing for ovarian tissue cryopreservation.

Item Type: Article
Uncontrolled Keywords: Cryopreservation; DNA damage; oocyte DNA; ovarian tissue; slow freezing; vitrification.
Subjects: Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Manipal
Date Deposited: 12 Jun 2014 04:31
Last Modified: 12 Jun 2014 04:31
URI: http://eprints.manipal.edu/id/eprint/139731

Actions (login required)

View Item View Item