Grape Seed Extract and Zinc Containing Nutritional Food Supplement Prevents Onset and Progression of Age-related Cataract in Wistar Rats

Satyam, Mani S and Bairy, KL and Pirasanthan, Rajadurai and Vaishnav, Lalit R (2014) Grape Seed Extract and Zinc Containing Nutritional Food Supplement Prevents Onset and Progression of Age-related Cataract in Wistar Rats. The Journal of Nutrition, Health & Aging, 18 (5). pp. 524-530.

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Abstract

Objective: To study possible inhibition of oxidative stress and cataract formation by single combined formulation of grape seed extract and Zincovit tablets against sodium selenite-induced age-related cataract in Wistar rat pups. Methods: Oxidative stress and consequent cataract formation was induced by subcutaneous administration of a single dose of sodium selenite (10 μmoles/kg) to Wistar rat pups on day 7 post-natally. In experiments designed to inhibit such cataract formation, the pups were pretreated subcutaneously with combined formulation of grape seed extract and Zincovit tablets (40, 80 and 160 mg/kg), one day prior to the administration of selenite and continuing such treatment till day 20, when the experiments were terminated. The extent of tissue damage caused by the selenite was assessed biochemically by measurements of the levels of reduced glutathione,glutathione peroxidase, glucose-6-phosphate dehydrogenase, protein thiol, catalase, superoxide dismutase, malondialdehyde, aldose reductase, sorb itol dehydrogenase and adenosine triphosphate in the isolated lenses. Cataract formation and its prevention were monitored by examining the eye with pen light illumination and subsequent photography of the isolated lenses. Results: Injection of selenite led to a significant loss of lens clarity due to cataract formation. In the group treated with combined formulation of grape seed extract and Zincovit tablets, the formation of cataract was significantly prevented. In the normal and selenite induced senile cataract control group, the levels of lens oxidative stress markers, G6PD and ATP were substantially lower than in the grape seed extract with Zincovit tablets treated group (p < 0.05). Conclusion: Over all, the results suggest that single combined formulation of grape seed extract and Zincovit tablets may offer a prophylactic measure against onset and progression of age- related cataract of human subjects as nutritional food supplement.

Item Type: Article
Uncontrolled Keywords: Selenite cataract; Oxidative stress; ATP; Grape seed extract; Zincovit tablets
Subjects: Medicine > MMMC Manipal > Pharmacology
Medicine > KMC Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 18 Jun 2014 04:27
Last Modified: 18 Jun 2014 04:27
URI: http://eprints.manipal.edu/id/eprint/139797

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