Study of association of angiotensin converting enzyme gene polymorphisms and target organ damage in type 2 diabetes mellitus

Chakraborty, Joydeep and Prabhu, Ravindra and Nagaraju, Shankar P and Bairy, Manohar and Satyamoorthy, K and Kosuru, Srinivas and Parthasarathy, Rajeevalochana (2014) Study of association of angiotensin converting enzyme gene polymorphisms and target organ damage in type 2 diabetes mellitus. Nephrology Dialysis Transplantation, 29 (Supl-3). pp. 419-433.

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Introduction and Aims: Diabetes mellitus is a global health problem. Insertion/ deletion (I/D) polymorphisms of angiotensin converting enzyme(ACE) gene are associated with ACE levels and increased risk of coronary heart disease but their association with other complications of diabetes mellitus has not been fully understood with conflicting results in various studies. We studied distribution of ACE gene polymorphisms and their relation to target organ damage in Type 2 Diabetes mellitus in our population. Methods: After ethics committee clearance, we prospectively did ACE gene polymorphism analysis by polymerase chain reaction, restriction fragment length polymorphism and DNA sequencing in patients of type 2 diabetes mellitus attending our nephrology clinic over 6 months. Clinical, demographic and biochemical data were collected. Association of various polymorphisms with complications of diabetes mellitus such as proteinuria, retinopathy, dyslipidemia, ischemic heart disease, peripheral neuropathy, hypertension and decline in glomerular filtration rate(GFR) were studied. Analysis was done on SPSS version 15 using using Pearsons Chi Square Test for comparing categorical variables and one way Anova to compare continuous variables. Results: Of 130 patients, 103 were males (79%), mean age 53.88 ± 9 years, average GFR of the study group was 53.88 ml/min/1.73 m2. The genotype distribution was I/D 58.5%, I/I 29.2% and DD 12.3%. None of the ACE gene polymorphisms were significantly associated with hypertension, proteinuria, retinopathy, peripheral neuropathy, dyslipidemia, ischemic heart disease, left ventricular systolic dysfunction; diastolic dysfunction, left ventricular hypertrophy and left ventricular ejection fraction (table 1). On follow up in 69 patients over 28.5 months interquartile range (IR) (12 to 44.7) the median GFR fall/month was not significantly different among the genotypes (table 2). Conclusions: ACE I/D gene polymorphisms are not associated with complications of diabetes mellitus and progression of diabetic nephropathy.

Item Type: Article
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Medicine
Medicine > KMC Manipal > Nephrology
Depositing User: KMC Manipal
Date Deposited: 19 Jun 2014 04:38
Last Modified: 19 Jun 2014 04:38

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