Investigation of In vitro, In vivo antioxidant and hepatoprotective activities of Eugenia jambolana Lam. stem bark

Rai, Padmalatha S (2011) Investigation of In vitro, In vivo antioxidant and hepatoprotective activities of Eugenia jambolana Lam. stem bark. Journal of Pharmacy Research, 4 (11). pp. 4167-4171. ISSN 0974-6943

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Abstract

Background: The world today is not restricted to the use of synthetic medicine but also focusing more upon the herbal medication for curing various ailments. The trend of validating the plants with traditional medicinal usage is increasing. Objective : The present investigation deals with the evaluation of antioxidant properties of Eugenia jambolana stem bark methanol extract and its effectiveness against liver intoxication. Materials and Mehods : In vitro antioxidant activity was evaluated using the parameters such as free radical scavenging using DPPH, Ferric ion reducing power, total antioxidant capacity and hydroxyl radical scavenging. The results were promising and prompted for further in vivo investigations by inducing liver damage in male Wistar rats using carbon tetrachloride. Result: Methanol extract of of stem bark of E. jambolana Lam. showed appreciable and concentration dependent in vitro antioxidant activity. In the in vivo evaluation, Pretreatment of methanolic extract (100 mg/kg and 200 mg/kg .w. p.o.) retained the normal liver architecture which was confirmed by assaying the serum non-specific enzymes SGOT, SGPT, ALP and total bilirubin. Also by the restoration of the activities of enzymes – superoxide dismutase, catalase and peroxidase and reduced lipid peroxidation it became evident that the plausible mechanism for hepatoprotective nature of the extract was the antioxidant activity.Conclusion: The study concludes that the stem bark methanolic extract of E. jambolana has potent hepatoprotective nature by the restoration of antioxidant enzyme levels.

Item Type: Article
Uncontrolled Keywords: Free radicals; scavengers; liver toxicity; herbal medication.
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 20 Jun 2014 10:06
Last Modified: 20 Jun 2014 10:06
URI: http://eprints.manipal.edu/id/eprint/139858

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