Evaluation of the Suitability of Ex Vivo Handled Ovarian Tissues for Optical Diagnosis by Raman Microspectroscopy

Murali Krishna, C and Sockalingum, GD and Venteo, L and Bhat, Rani A and Kushtagi, Pralhad and Pluot, M and Manfait, M (2005) Evaluation of the Suitability of Ex Vivo Handled Ovarian Tissues for Optical Diagnosis by Raman Microspectroscopy. Biopolymers, 79. pp. 269-276.

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A pilot Raman microspectroscopy study of formalin-fixed, paraffin-embedded, and deparaffinized sections from the same ovarian normal and malignant tissues was carried out. This approach was considered in order to evaluate the suitability of these ex vivo tissue handling procedures in discrimination as well as biochemical characterization. The spectra of formalin-fixed normal and malignant tissues exhibited no contamination due to formalin, which is indicated by the absence of strong formalin peaks; spectral features also show significant differences for normal and malignant tissues. The differences between spectral profiles of deparaffinized normal and malignant tissues are subtle and spectra show few residual sharp peaks of paraffin. Complete dominance of paraffin swamping signals from tissues was observed in the spectra of paraffin-embedded tissues. Principal components analysis (PCA), which was employed for discrimination of tissue type, provided good discrimination for formalin-fixed and paraffin-embedded tissue spectra. PCA of deparaf-

Item Type: Article
Uncontrolled Keywords: Micro-Raman spectroscopy, ovarian tissues, tissue handling, characterization,
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Manipal
Date Deposited: 30 Sep 2011 04:21
Last Modified: 30 Sep 2011 04:21
URI: http://eprints.manipal.edu/id/eprint/1399

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